Research

Publications

K.N. Lam, J. Cheng, K. Engel, J.D. Neufeld, T.C. Charles (2015). Current and future resources for functional metagenomics.
[PDF] [Pubmed] [Journal]

K.N. Lam, T.C. Charles (2015). Strong spurious transcription likely contributes to DNA insert bias in typical metagenomic clone libraries.
[PDF] [Pubmed] [Journal] [bioRxiv]

H.S. Najafabadi, S. Mnaimneh, F.W. Schmitges, M. Garton, K.N. Lam, A. Yang, M. Albu, M.T. Weirauch, E. Radovani, P.M. Kim, J. Greenblatt, B.J. Frey, T.R. Hughes (2015). C2H2 zinc fingers greatly expand the human regulatory lexicon.
[PDF] [PubMed] [Journal]

K.N. Lam, M.W. Hall, K. Engel, G. Vey, J. Cheng, J.D. Neufeld, T.C. Charles (2014). Evaluation of a pooled strategy for high-throughput sequencing of cosmid clones from metagenomic libraries.
[PDF] [PubMed] [Journal]

K.N. Lam, H. van Bakel, A.G. Cote, A. van der Ven, T.R. Hughes (2011). Sequence specificity is obtained from the majority of modular C2H2 zinc finger arrays.
[PDF] [PubMed] [Journal]

M.A. Trainer, D.S. Capstick, A. Zachertowska, K.N. Lam, S.R.D. Clark, T.C. Charles (2010). Identification and characterization of the intracellular poly-3-hydroxybutyrate depolymerase PhaZ of Sinorhizobium meliloti.
[PDF] [PubMed] [Journal]

Selected Poster Presentations

K.N. Lam, E.C. Martens, and T.C. Charles (2015). Developing Bacteroides thetaiotaomicron as a host for function-based screening of human gut metagenomic DNA. Presented at the Canadian Society for Microbiologists Annual Meeting. Regina, SK, Canada.
[PDF]

K.N. Lam and T.C. Charles (2014). GC bias in metagenomic cosmid libraries may be due to selection against transcriptionally active fragments in E. coli. Presented at the Argonne Soil Metagenomics Meeting. St. Charles, IL, USA.
[PDF]

T.C. Charles, J. Cheng, K.N. Lam, K. Engel, T. Romantsov, and J.D. Neufeld (2012). Strategies for screening metagenomic libraries in multiple surrogate hosts. Presented at the International Society for Microbial Ecology Annual Meeting. Copenhagen, Denmark.

C. Manassieva, K.N. Lam, J. Cheng, and T.C. Charles (2011). Functional screening of soil metagenomic libraries for bacterial conjugation genes. Presented at the Argonne Soil Metagenomics Workshop. Bloomingdale, IL, USA.

K.N. Lam, J.L. McGuire, J. Cheng, and T.C. Charles (2011). Cloning high-molecular-weight and plasmid DNA from the human gut microbiome for functional screening. Presented at the American Society of Microbiology General Meeting. New Orleans, LA, USA.
[PDF]

K.N. Lam, J.L. McGuire, J. Cheng, and T.C. Charles (2011). Snoop through poop: Cloning DNA from the human gut microbiome for function-based screening. Presented at the University of Waterloo Graduate Student Research Conference. Waterloo, ON, Canada.
[PDF]

K.N. Lam and T.R. Hughes (2010). C2H2 zinc finger modules typically retain sequence specificity in modular assemblies. Presented at the Cold Spring Harbour Laboratory Meeting on Systems Biology: Global Regulation of Gene Expression. Cold Spring Harbor, NY, USA.

Selected Talks

A comparison of pooled and barcoded approaches for Illumina DNA sequencing of large-insert clones from metagenomic libraries. Student Award Symposium, Conference of the Canadian Society of Microbiologists. Ottawa, ON. June 19, 2013.
[SlideShare] [ISME 8 Symposium Award]

Theses

Development and analysis of molecular methods for functional metagenomics of the human gut microbiome. Ph.D. Thesis.
[PDF] [Repository]

A phage display system to profile the DNA-binding specificities of C2H2 zinc fingers. M.Sc. Thesis.
[PDF] [Repository]